Diabetes is now reaching epidemic proportions, and the associated complications of this disease can be disabling and even life-threatening. In Type 2 Diabetes: Methods and Protocols, leading investigators provide up-to-date explanations of commonly used laboratory protocols used in diabetes research. Covering the commonly described in vivo and in vitro model systems, the volume ultimately leads to an overall view of how cellular dysfunction and degeneration leads to susceptibility and diabetes disease progression. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and expert notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Type 2 Diabetes: Methods and Protocols offers succinct, proven techniques to aid research scientists and clinicians in continuing the study of this debilitating disease.
1) Selecting the 'Right' Mouse Model for Metabolic Syndrome and Type 2 Diabetes Research Edward H. Leiter 2) Nutritional Models of Type 2 Diabetes Mellitus Beverly Sara Muhlhausler 3) The Isolation and Purification of Rodent Pancreatic Islets of Langerhans Jacqueline F. O'Dowd 4) The Measurement of Insulin Secretion from Isolated Rodent Islets of Langerhans Anna L. Nolan and Jacqueline F. O'Dowd 5) The Incubation and Monitoring of Cell Viability in Primary Rat Islets of Langerhans and Pancreatic ss-Cell Lines Noel G. Morgan, Eleftheria Diakogiannaki, and Mark A. Russell 6) In Vitro Culture of Isolated Islets of Langerhans: Analysis of Function Anna L. Nolan 7) Single Cell RT-PCR Identification of Genes Expressed by Human Islet Endocrine Cells Dany Muller, Peter M. Jones, and Shanta J. Persaud 8) Laser Capture Microdissection of Human Pancreatic b -Cells and RNA Preparation for Gene Expression Profiling Lorella Marselli, Dennis C. Sgroi, Susan Bonner-Weir, and Gordon C. Weir 9) In Vitro Transdifferentiation of Human Hepatoma Cells into Pancreatic-Like Cells Wan-chun Li 10) The Measurement of GLUT4 Translocation in 3T3-L1 Adipocytes Nicky Konstantopoulos and Juan Carlos Molero-Navajas 11) Pancreatic Remodeling: Beta Cell Apoptosis, Proliferation and Neogenesis and the Measurement of Beta Cell Mass and of Individual Beta Cell Size Eduard Montanya and Noelia Tellez 12) Morphology of Pancreatic Islets: A Time Course of Pre-Diabetes in Zucker Fatty Rats Petra Augstein and Eckhard Salzsieder 13) Fluorescent Immunohistochemistry and in situ Hybridization Analysis of Pancreas Xiuli Wang, Shundi Ge, and Gay M. Crooks 14) The Measurement of Insulin Secretion Using Pancreas Perfusionin the Rodent Edward T. Wargent 15) Hyperinsulinemic-Euglycemic Clamp to Assess Insulin Sensitivity In Vivo Jason K. Kim 16) Gene Expression Analysis in Diabetes Research Peter White and Klaus H. Kaestner 17) Gene Expression Mining in Type 2 Diabetes Research Donald R. Dunbar