Tecniche di laboratorio

Metodologie biochimiche è destinato agli studenti che si avvicinano per la prima volta alle tecniche - molto numerose e spesso

Metodologie biochimiche e biomolecolari permette di acquisire la padronanza dei principali metodi usati nei laboratori per l'a

RNA Methodologies, Fifth Edition continues its tradition of excellence in providing the most up-to-date ribonucleic acid lab techniques for seasoned scientists and graduate students alike. This edition features new material on the exploding field of microRNA as well as the methods for the profiling of gene expression, both which have changed considerably in recent years. As a leader in the field, Dr. Farrell provides a wealth of knowledge on the topic of RNA while also giving readers helpful hints from his own personal experience in this subject area. Beginning with the most contemporary, RNA Methodologies, Fifth Edition, presents the essential techniques to use when working with RNA for the experienced practitioner while at the same time providing images and examples to aid the beginner in fully understanding this important branch of molecular biology. The next generation of scientists can look to this work as a guide for ensuring high productivity and highly representative data, as well as best practices in troubleshooting laboratory problems when they arise. Features new material in miRNA, MIQE guidelines, biomarkers, RNA sequencing, digital PCR and more Includes expanded coverage on quantitative PCR techniques, RNAi, bioinformatics, the role of locked nucleic acids, aptamer biology, PCR arrays, and other modern technologies Presents comprehensive, cutting-edge information covering all aspects of working with RNA Builds from basic information on RNA techniques to in-depth protocols to guidance on how to modify and adjust each step of a particular application Presents multiple avenues for addressing the same experimental goals

This is a quick reference guide to the selection and interpretation of more than 450 commonly used diagnostic tests. It covers: basic principles of diagnostic testing, common blood, urine and cerebrospinal fluid laboratory tests, therapeutic drug monitoring, microbiologic test selection and interpretation and diagnostic imaging tests by body system, electrocardiography, and differential diagnosis tables & algorithms; and tests used in internal medicine, pediatrics, surgery, neurology and obstetrics and gynecology. It includes: costs and risks of diagnostic tests; evidence-based information; diseases associated with abnormal test results, including test sensitivities; full literature citations with PubMed (PMID) numbers included for each reference; more than 24 NEW clinical laboratory test entries, 6 NEW differential diagnosis tables; 5 NEW diagnostic algorithms; and, NEW sections on point-of-care testing, provider-performed microscopy, pharmacogenetic testing, and diagnostic echocardiography.

Among animals used in research, teaching and testing, mice are now widely recognized as the most important model for human diseases and disorders. They comprise the majority of all experimental mammals and tend to be the model of choice used for research into many diseases/disorders including cancer, heart disease, asthma, Alzheimer's, Down syndrome, deafness, osteoporosis, obesity, diabetes and even mental health research. Additionally the laboratory mouse continues to play a widely publicized vital role in the human genome project. One of the most time-consuming activities in research laboratories is looking up information specific to the species or strain of animal being used. This book, part of the highly successful "Handbook of Experimental Animals" series, allows the user quick access to any point of interest on the mouse as an experimental model. It is edited by Hans Hedrich, Hannover Medical School. It is a comprehensive reference source written by international experts. It is well-illustrated with high quality detailed images. It features a two-color, user-friendly format combined with color plate sections.

Con questo volume la Sltl (Società Italiana di Igiene, Medicina Preventiva e Sanità Pubblica) vuole contribuire a dare la massima diffusione, fra i soci e fra tutti coloro che operano nel campo della Sanità pubblica, alle possibilità di applicazione delle moderne tecniche di laboratorio a sostegno delle indagini epidemiologiche e degli interventi di prevenzione. Negli ultimi anni, infatti, le metodologie biologiche-molecolari si sono imposte come strumento di indiscutibile importanza sia in campo diagnostico che nella ricerca scientifica.