Several anti-angiogenic agents are now in clinical trials for a range of diseases characterized by uncontrolled blood vessel formation, including cancer and diabetic retinopathy. The potential value of anti-angiogenic agents has given researchers in this field new enthusiasm and impetus. This volume will provide a set of up-to-date reviews on the important components of this process coupled with a set of critical chapters describing the various techniques for studying angiogenesis. In addition, several state-of-the-art chapters will describe associated techniques such as the isolation of microvascular endothelial cells, and the basic principles behind the use of transgenic mice in the study of angiogenesis. The book will provide not only 'recipes' for the study of angiogenesis, but also an up-to-date account of the biology of angiogenesis.
Indice testuale
I. Special Review Article. Therapeutic Inhibition of Angiogenesis, Hua-Tang Zhang and Roy Bicknell. II. Angiogenesis Protocols In Vivo. Microscopic Assessment of Angiogenesis in Tumors, Stephen B. Fox. In Vivo Matrigel Migration and Angiogenesis Assays, Katherine M. Malinda. Alginate Microbead Release Assay of Angiogenesis, Clifford Y. Ko, Vivek Dixit, William W. Shaw, and Gary Gitnick. Disc Angiogenesis Assay, Anthony C. Allison and Luis-F. Fajardo. Sponge Implant Model of Angiogenesis, Silvia P. Andrade. Hollow Fiber Assay for Tumor Angiogenesis, Roger M. Phillips and Michael C. Bibby. Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy, Axel Sckell and Michael Leunig. Angiogenesis Assays Using Chick Chorioallantoic Membrane, David C. West, W. Douglas Thompson, Paula G. Sells, and Mike F. Burbridge. Corneal Assay for Angiogenesis, Marina Ziche. III. Angiogenesis Protocols In Vitro. Collagen Gel Assay for Angiogenesis: Induction of Endothelial Cell Sprouting, Ana M. Schor, Ian Ellis, and Seth L. Schor. Chemotaxis and Chemokinesis in 3D Macromolecular Matrices: Relevance to Angiogenesis, Ana M. Schor, Ian Ellis, and Seth L. Schor. Rat Aortic Ring: 3D Model of Angiogenesis In Vitro, Mike F. Burbridge and David C. West. In Vitro Matrigel Angiogenesis Assays, M. Lourdes Ponce. IV. Associated Techniques. Microvessel Endothelial Cells from Human Adipose Tissues: Isolation, Identification, and Culture, Peter W. Hewett. Transfection and Transduction of Primary Human Endothelial Cells, Stewart G. Martin. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization, Richard C. M. Siow and Jeremy D. Pearson. Bovine Retinal Microvascular Pericytes: Isolation, Propagation, and Identification, Ramesh C. Nayak and Ira M. Herman. Index.
