Pancreatic ductal adenocarcinoma is the fourth leading cause of cancer death in the United States. Annually approximately 30,000 Americans are diagnosed with the disease and most will die from it within five years. P- creatic ductal adenocarcinoma is unique because of its late onset in age, high mortality, small tumor samples infiltrated with normal cells, and a lack of both early detection and effective therapies. Some of these characteristics have made studying this disease a challenge. Pancreatic cancer develops as a result of the accumulation of genetic alterations in cancer-causing genes, such as the oncogenes and the tumor-s- pressor genes. In the last decade, major progress has been made in identifying important oncogenes and tumor-suppressor genes for the disease. In Panc- atic Cancer: Methods and Protocols, we review the classical techniques that have contributed to the advances in pancreatic research and introduce new strategies that we hope will add to future breakthroughs in the field of cancer biology.
Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of s- radic and familial pancreatic cancer. It covers topics from in vitro cell c- tures to in vivo mouse models, DNA to protein manipulation, and mutation analyses to treatment development. We believe that our book will prove an invaluable source of proven protocols for those who are interested in either basic or translational research in pancreatic cancer.
Identification and Analysis of Precursors to Invasive Pancreatic Cancer Ralph H. Hruban, Robb E. Wilentz, and Anirban Maitra Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical Applications G. Steven Bova, Isam A. Eltoum, John A. Kiernan, Gene P. Siegal, Andra R. Frost, Carolyn J. M. Best, John W. Gillespie, and Michael R. Emmert-Buck Immunohistochemistry and In Situ Hybridization in Pancreatic Neoplasia Robb E. Wilentz, Ayman Rahman, Pedram Argani, and Christine Iacobuzio-Donahue Practical Methods for Tissue Microarray Construction Helen L. Fedor and Angelo M. De Marzo Xenografting and Harvesting Human Ductal Pancreatic Adenocarcinomas for DNA Analysis Kimberly Walter, James Eshleman, and Michael Goggins Culture and Immortalization of Pancreatic Ductal Epithelial Cells Terence Lawson, Michel Ouellette, Carol Kolar, and Michael Hollingsworth DNA Methylation Analysis in Human Cancer Carmelle D. Curtis and Michael Goggins Digital Single-Nucleotide Polymorphism Analysis for Allelic Imbalance Hsueh-Wei Chang and Ie-Ming Shih Representational Difference Analysis as a Tool in the Search for New Tumor Suppressor Genes Antoinette Hollestelle and Mieke Schutte Serial Analyses of Gene Expression (SAGE) Jia Le Dai Oligonucleotide-Directed Microarray Gene Profiling of Pancreatic Adenocarcinoma David E. Misek, Rork Kuick, Samir M. Hanash, and Craig D. Logsdon Identification of Differentially Expressed Proteins in Pancreatic Cancer Using a Global Proteomic Approach Christophe Rosty and Michael Goggins Detection of Telomerase Activity in Patients with Pancreatic Cancer Kazuhiro Mizumoto and Masao Tanaka Serological Analysis of Expression cDNA Libraries (SEREX): An Immunoscreening Technique for Identifying Immunogenic Tumor Antigens Yao-Tseng Chen, Ali O. Gure, and Matthew J. Scanlan Modeling Pancreatic Cancer in Animals to Address Specific Hypotheses Paul J. Grippo and Eric P. Sandgren Strategies for the Use of Site-Specific Recombinases in Genome Engineering Julie R. Jones, Kathy D. Shelton, and Mark A. Magnuson Primary Explant Cultures of Adult and Embryonic Pancreas Farzad Esni, Yoshiharu Miyamoto, Steven D. Leach, and Bidyut Ghosh Zebrafish as a Model for Pancreatic Cancer Research Nelson S. Yee and Michael Pack Development of a Cytokine-Modified Allogeneic Whole Cell Pancreatic Cancer Vaccine Dan Laheru, Barbara Biedrzycki, Amy M. Thomas, and Elizabeth M. Jaffee Overview of Linkage Analysis: Application to Pancreatic Cancer Alison P. Klein Index