Prostate cancer is the second leading cancer in men in Western society. A major concern, and an area of intensive research, involves understanding why certain prostate cancers remain localized or indolent, whereas others become aggressive and metastasize. The differences between these cancer types have profound implications for patients and physicians. Indolent d- ease, which grows very slowly, generally does not cause any problems to the patient, whereas aggressive disease requires immediate treatment, the earlier the better. At present, there are no markers that discriminate between these two entities, thus causing a dilemma for the management of patients who have recently been diagnosed. The aim of Prostate Cancer Methods and Protocols is to explore cutting-edge molecular methods that may have the potential to reveal markers of disease for use in more accurate diagnoses of prostate c- cer and, consequently, to lead to new treatment strategies.
This book provides a comprehensive collection of both in vitro and in vivo step-by-step protocols currently used by leaders in prostate cancer research, advice on approaches that can be used in the study of prostate cancer, as well as reviews covering areas less amenable to laboratory research, such as environmental factors in prostate cancer, to provide the reader with an overview of the prostate cancer research field as it currently stands.
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Epidemiological Investigation of Prostate Cancer Graham G. Giles Human Prostate Cancer Cell Lines Pamela J. Russell and Elizabeth A. Kingsley Growth of Prostatic Epithelial and Stromal Cells In Vitro Donna M. Peehl Prostate Epithelial Stem Cell Isolation and Culture David L. Hudson and John R. W. Masters Generation of Immortal Human Prostate Cell Lines for the Study of Prostate Cancer Johng S. Rhim Spheroids of Prostate Tumor Cell Lines George Sgouros, Wei-Hong Yang, and Richard Enmon Animal Models of Prostate Cancer Pamela J. Russell and Dale J. Voeks Transgenic Mouse Models for Prostate Cancer: Identification of an Androgen-Dependent Promoter and Creation and Characterization of the Long Probasin Promoter-Large T Antigen (LPB-Tag) Model Susan Kasper, William Tu, Richard L. Roberts, and Scott B. Shappell In Vivo Models of Human Prostate Cancer Bone Metastasis Julie M. Brown Effects of Fixation on Tissues Elin Mortensen and Julie M. Brown Background, Methods, and Protocols for the Histopathological Diagnosis of Prostate Carcinoma Warick Delprado Realizing the Potential of Ejaculate/Seminal Fluid in Detecting and Predicting Natural History R. A. Gardiner, Michelle Burger, Judith A. Clements, and Martin F. Lavin Bisulfite Methylation Analysis of Tumor Suppressor Genes in Prostate Cancer from Fresh and Archival Tissue Samples Susan J. Clark, Douglas S. Millar, and Peter Molloy Production and Characterization of Antipeptide Kallikrein 4 Antibodies: Use of Computer Modeling to Design Peptides Specific to Kallikrein 4 Tracey J. Harvey, Ying Dong, Loan Bui, Russell Jarrott, Terry Walsh, and Judith A. Clements The Androgen Receptor CAG Repeat and Prostate Cancer Risk Peter E. Clark, Ryan A. Irvine, and Gerhard A. Coetzee Studies on Androgen Receptor Mutations and Amplification in Human Prostate Cancer Zoran Culig, Alfred Hobisch, Martin Erdel, Georg Bartsch, and Helmut Klocker Proteomics in the Analysis of Prostate Cancer Soren Naaby-Hansen, Kohji Nagano, Piers Gaffney, John R. W. Masters, and Rainer Cramer Application of Gene Microarrays in the Study of Prostate Cancer Colleen C. Nelson, Douglas Hoffart, Martin E. Gleave, and Paul S. Rennie Enhancer Trap Method Using a Green Fluorescent Protein Reporter Plasmid for Cloning Tissue-Specific Enhancers Active in Prostate Cells Fujiko Watt and Peter Molloy Targeted Alpha Therapy of Prostate Cancer Barry J. Allen, Yong Li, Syed M. A. Rizvi, and Pamela J. Russell Phenotypic and Functional Differences of Dendritic Cells Generated Under Different In Vitro Conditions Stephanie E. B. McArdle, Selman A. Ali, Geng Li, Shahid Mian, and Robert C. Rees Flavonoid Compounds in the Prevention and Treatment of Prostate Cancer Graham E. Kelly and Alan J. Husband Index
