Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperienced immunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well as purification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonal antibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.
Indice testuale
Preparation of Rodent Monoclonal Antibodies by in vitro Somatic Hybridization ; Preparation of Recombinant Antibodies From Immune Rodent Spleens and the Design of Their Humanisation by CDR Grafting ; Selection of Antibodies From Phage Libraries of Immunoglobulin Genes ; Arm Complexes for in vitro Display and Evolution of Antibody Combining Sites ; Human Monoclonal Antibodies to Blood Group Antigens ; Laboratory Based Methods for Small-Scale Productions of Monoclonal Antibodies ; Isolation and Purification of Antibodies From Tissue Culture Supernatant ; Antibody Production in Plants ; Radiolabelling of Monoclonal Antibodies ; Non-Radioactive Antibody Probes ; Immunogold Probes for Light and Electron Microscopy ; Characterization of Cellular Antigens Using Monoclonal Antibodies ; Immunoassays ; Immunoaffinity Chromatography of Macromolecules ; Immunochemical Detection of BrdUrd-Labelled Nuclei for Monitoring Cell Kinetics ; Immunofluorescence Microscopy ; Analysis of Clinical Haematological Samples in Transplantation for Cancer ; Immunocytochemical Staining of Cells and Tissues for Diagnostic Applications ; Detection of Chemically Modified DNA in Lymphocytes of Patients Undergoing Chemotheraphy ; Monoclonal Antibody Therapy in Organ Transplantation ; Monoclonal Antibody Therapy in Rheumatoid Arthritis
