"A wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years." - Pharmaceutical Research. This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.
Indice testuale
Contributors. Preface (R. Freshney & M. Freshney). Preface to the First Edition (R. Freshney). List of Abbreviations. Introduction (R. Freshney). Cell Interaction and Epithelial Differentiation (N. Maas Szabowski, et al.). The Epidermis (E. Parkinson & W. Yeudall). Culture of Human Mammary Epithelial Cells (M. Stampfer, et al.). Culture of Human Cervical Epithelial Cells (M. Stanley). Human Prostatic Epithelial Cells (D. Peehl). Human Oral Epithelium (R. Grafstrom). Normal Human Bronchial Epithelial Cell Culture (J. Wise & J. Lechner). Isolation and Culture of Pulmonary Alveolar Epithelial Type II Cells (L. Dobbs & R. Gonzalez). Isolation and Culture of Intestinal Epithelial Cells (C. Booth & J. O Shea). Isolation and Culture of Animal and Human Hepatocytes (C. Guguen Guillouzo). Culture of Human Urothelium (J. Southgate, et al.). Other Epithelial Cells (R. Freshney). List of Suppliers. Index.
